Gamma-tocotrienol treatment increased peroxiredoxin-4 expression in HepG2 liver cancer cell line
نویسندگان
چکیده
BACKGROUND To determine the antiproliferative effect of gamma-tocotrienol (GTT) treatment on differential protein expression in HepG2 cells. METHODS HepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS GTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. One of these upregulated proteins was identified as peroxiredoxin-4 (Prx4). Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment. CONCLUSIONS GTT might directly influence the expression dynamics of peroxiredoxin-4 to control proliferation in liver cancer.
منابع مشابه
Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferation
Background: Liver cancer is the second outstanding reason for cancer-related death global and thus a main public health challenge. Plant-based drugs are regarded as promising therapies. Basil and Dracocephalum kotschyi extracts have shown therapeutic impacts on various cancers. Therefore, we assessed the effects of the extract of Basil and Dracocephalum kotschyi on cell proliferation and BCL2 e...
متن کاملEffects of fibromodulin protein expression on NFkB and TGFβ signaling pathways in liver cancer cells
Introduction: The incidence rate of liver cancer is continuously increasing. Currently, gene therapy is applied to improve various medical issues such as cancer treatment approaches. Correspondingly, fibromodulin involves in many biological and physiological processes through interaction with growth factors and signaling pathway receptors. The aim of this study was to investigate the effects of...
متن کاملAnti-Proliferative, Anti-Oxidant and Pro-apoptotic Effect of Alcoholic Extract of lavender in HepG2 cell lines
Background: Liver cancer is the second leading cause of cancer deaths in the world and is therefore a major public health challenge. In recent years, many of the drugs used in the treatment of many malignancies and cancers have been of plant origin. Lavender extract has been shown to have therapeutic effects in various cancers. Therefore, the biological effects of ethanolic extract of lavender ...
متن کاملSynthesis of Titanium Oxide Nanoparticles Functionalized by Glutamine and Conjugated to Thiosemicarbazide and their Effect on the Expression of the CASP3, Bcl2, and BAX genes
Introduction: The increasing trend of cancer morbidity and mortality is a major human health concern, indicating the necessity for the design and introduction of novel anticancer compounds. The use of nanotechnology products is a new approach to cancer treatment. Therefore, the current study was performed to synthesize Titanium Dioxide nanoparticles (NPs) functionalized with glutamine and conju...
متن کاملChitosan extracted from the Persian Gulf chiton shells: Induction of apoptosis in liver cancer cell line
Here for the first time, we investigated the cytotoxic effects of the chitosan extracted from the Persian Gulf Chiton shell (Acanthopleura vaillantii) on liver cancer cell line (HepG2). Chitosan extraction was implemented following this method: chitin was produced by demineralization and deproteinization procedure, and the extracted chitin was converted into soluble chitosan using deacetylation...
متن کامل